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KMID : 0861020080230030067
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Korea Journal of Herbology
2008 Volume.23 No. 3 p.67 ~ p.72
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Determination of Rosmarinic Acid and Caffeic Acid from Perilla frutescens var. japonica and var. acuta by Reversed-Phase HPLC
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Kim Byung-Youn
Jeong Ji-Seon
Kwon Ha-Jeong
Lee Je-Hyun
Hong Seon-Pyo
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Abstract
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Objectives : This study presents a high performance liquid chromatography methods for the quantitative and qualitative analysis of rosmarinic acid (RA) and caffeic acid (CA) in Perilla frutescens var. japonica and var. acuta.
Methods : Chromatographic separation was performed using a mixture of methanol, water and formic acid (35 : 64.2 : 0.8) with a reversed-phase column (Gemini C18, 4.6 × 150 mm, 3 ㎛). The analyses were detected at UV (280 nm).
Results : The samples were extracted with 50% EtOH under reflux for 1 h, and simultaneous determination for RA and CA in hyang-so-san and haeng-so-san was possible without interference peaks
Conclusions : According the results, we developed a determination method for RA and CA in Perillae Folium. Owing to Perilla frutescens var. japonica and var. acuta did not show significant difference in contents of RA and CA, both Perilla frutescens could be available as herbal medicine.
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KEYWORD
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Perilla frutescens, Perillae Folium, Rosmarinic acid, Caffeic acid
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